Coding

Part:BBa_K1129013:Design

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-08-30)


CaXMT1 under arabinose-induced promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR amplification was initially difficult because we had designed quite long primers to add the standard prefix (which included the bacterial consensus sequence) and suffix.


Source

Modified from 2012 TU Munich iGEM Part: BBa_K801070.

References